Shanghai Gudu solves the problem of Elisa test series for you
Many people in the elisa kit (enzyme-linked immunoassay kit) experiment, there will always be more or less problems, such as the curve problem or the high recovery rate, low, etc., Shanghai Gu Duo is now for you Unravel the questions in this series so that you can successfully detect the results while you are doing the experiment.
Curve problem:
1. The OD value is not normal:
The kit is not warmed and the kit is warmed to 25 ° C.
Temperature control is not good, control the correct temperature
Incubation time is not accurate, control incubation time
When washing, the impact force is too large, the soaking time is too long, the washing time is increased, washing 4-5 times, 250ul per hole, then patted in direct sunlight, directly blowing against the wind, avoiding the wind and avoiding the wavelength of the microplate reader, the enzyme label Dilution error of 450nm antibody at the instrument, correct dilution of antibody
Water quality problem, replaced with Wahaha mineral water
2. Whiteboard:
The washing liquid is incorrectly prepared, the correct preparation of the washing liquid is less liquid (missing liquid), the correct step is to add liquid
3. No gradient:
The standard product is also polluted. The OD value of the standard point plate is not normal. The temperature control time is to make an artificial point board.
4. The linearity is not good:
The OD value is not normal, and the temperature control time is done once.
Recovery rate:
1. Blank tube is positive:
The sample itself is a positive sample, and the negative sample is used as a recovery rate. The blank tube is contaminated in the experiment, and the cross-contamination is prevented in the experiment.
2. High:
The addition amount is inaccurate, the precise addition amount is not suitable for adding the concentration, and the appropriate concentration is added to take more dry liquid. The accurate liquid extraction is carried out to take other phase liquids, and the phase-blown dry solution is not diluted or added. Small amount, correct dilution solution
3. Low:
Inaccurate addition amount, accurate addition amount is not suitable for adding concentration, adding appropriate concentration to take liquid to dry less, accurate liquid extraction and drying solution dilution error or addition amount, correct dilution complex solution false negative, positive:
1, water quality reasons:
Point solution verification, replace with Wahaha mineral water
2, experimental operation reasons:
Pipetting to other phases to blow dry, accurate liquid extraction and drying is not thorough, prolonging the contamination of the sample during centrifugation, replacing the sample with emulsified untreated completely, and immersing in a warm bath (70 ° C)
3,
instrument
Reagent reasons:
The centrifuge tube is not cleaned, the effect of the organic solvent of the correct washing device, and the reagent blank will affect the result.
4, derivatization reagent reasons:
(long-term derivatization reagent deterioration), replacement of derivatization reagent
5, related to the curve is good or bad, to ensure the normal curve.
Shanghai Gudu provides free testing service: use Shanghai Guduo Elisa kit, only charge for the kit, all other auxiliary reagents are free. According to the experimental workload and difficulty level, Shanghai Guduo will agree on the experimental cycle, complete the entrusted experiment with quality and quantity, and send the experimental results and materials to you in free by express mail. Welcome to order!
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