Teach you a few tricks, how to identify fake and inferior ELISA kits
Recently, many customers have told us some companies and small workshops that are profit-seeking. They use fake and inferior ELISA kits to harm the vast number of scientific research workers. They often profit from the rigor and seriousness of scientific research, ignoring the rigor and seriousness of scientific research. Seriously disrupted the scientific research work and seriously disrupted the market order. Therefore, we have the obligation to expose these fake counterfeit ELISA kits and provide methods for identifying these counterfeit ELISA kits for the majority. Researchers and users refer to let those who sell sheep meat, fake imported reagents, and no integrity, fake fakes, such as the rats crossing the street, everyone shouted.
Identification method:
I. Use the interference test to identify whether the ELISA kit detects the antigen of interest, as follows:
(1) The kit-specific recombinant protein antigen and the detection antibody in the kit are configured as a mixed incubation solution of antibody:antigen≈1:2 (2) after incubation at 37 ° C for 15 minutes, instead of the detection in the original kit The subsequent operation of the antibody (3) is carried out according to the kit instructions, and the detection antibody, the enzyme complex and the substrate are added. (4) After the experiment is completed, the standard curve is detected. If the standard curve is good, the added target antigen and the kit antibody do not match. The kit detection antibody is directed to a non-target antigen, and the kit is a counterfeit ELISA kit.
(5) If the standard curve is wireless, it means that the detection antibody of the kit has been neutralized or interfered by the antigen of interest, which affects the detection of the actual kit antibody, and the kit detects that the antibody targets the antigen of interest.
II. If you have purchased two boxes of the same company's ELISA kits A and B, you can use the cross-over experiment to identify whether the ELISA kit is fraudulent, as follows:
(1) Take out two of the coated plates of the purchased kit A.
(2) A standard curve of a coated plate plus kit A is used as a standard curve.
(3) Another standard of the coated plate plus kit B is used as a standard curve.
(4) Follow-up operation is carried out according to the instructions of the kit, and the detection antibody, enzyme complex and substrate are added.
(5) After the experiment is completed, two standard curves are detected. If the two standard curves are identical, the same index detected by the two ELISA kits is not the A and B, that is, the kit is a counterfeit ELISA kit.
(6) If the two standard curves are inconsistent, the two indicators of the two ELISA kits are different. The reagent A can only detect A, and can not detect B, that is, the kit is a normal ELISA kit.
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