Application of Microscopic Analysis System in Microscope Inspection of Feed
Feed microscopy is based on animal and plant morphology and histocytology. With the help of a microscope, the external morphological characteristics of the sample to be tested (such as shape, color, particle size, hardness, etc.), tissue and cell structure characteristics and different staining characteristics A method for identifying and evaluating the type and quality of feed samples.
Microscopic image analysis system is composed of high-resolution color CMOS, powerful microscopic image analysis software and microscope. Professional image processing algorithms, super functions such as analysis, processing, measurement, editing, etc., widely used in micro cell image analysis, dust particle detection, animal and plant microstructure observation, fiber material research, jewelry identification and other fields.
1. Detection of microorganisms in feed
Commonly used detection methods for microorganisms include blood cell counting plate method, plate culture counting method, maximum likelihood counting method (MPN), enzyme-linked immunoassay (ELISA), DNA probe detection method (DNA Probe), polymerase chain reaction (PCR ) Etc., where the hemocytometer counts the microorganisms directly under the microscope, all other methods must use the microscope from the aspects of cell size, shape, spores, flagella, fimbria, capsule and Gram staining Identification. Whether it is the detection of pathogenic microorganisms in feed, such as Salmonella, Shigella, or beneficial microorganisms in microbial feed additives, such as Lactobacillus acidophilus and active yeast, all need to use a microscope.
2. Inspection of feed quality
For feed mills, farms and feed monitoring departments, how to quickly identify and evaluate feed materials, especially animal feed quality and adulterants, is an important part of people's concern. The microscopic examination of feed is an ideal method. Its main features are simple, fast and accurate. In particular, the accurate analysis of the raw material composition makes up for the shortcomings of the conventional chemical analysis, and makes a satisfactory evaluation of feed quality in a relatively short time.
2.1 The purity of a feed can be tested, and qualitatively detect pollutants and adulterants, especially some toxic and harmful ingredients that seriously affect the quality of feed. Through screening, separation, flotation and other means, microscopic examination can roughly determine the quantitative ratio of a certain substance. For example, when checking whether the sundries in fish meal and meat and bone meal cakes exceed the allowable range, this method is often used for identification.
2.2 Used for qualitative identification of pre-mixed feed, trace elements, vitamins and antibiotic drug additives.
2.3 Through the detection of the degree of contamination of feed by bacteria, fungi, ho, etc., microscope detection technology can provide information on the freshness of feed.
2.4 Through the acid-alkali treatment, the microscopic inspection technique can analyze the cell structure of the dopant in the sample to be accurately characterized. For example: when fish meal contains feather powder, rice husk, cottonseed cake, sawdust and other adulterants, this method is often used for identification.
2.5 Used to identify fake and shoddy goods. With the rapid development of animal husbandry and feed industry, feed protein resources are increasingly scarce. At present, many high-protein waiting, (such as fish meal, meat and bone meal, yeast, etc.) adulteration phenomenon occurs from time to time. Microscope detection technology can easily detect adulterants through the morphology, cell structure and staining characteristics of word materials, identify the authenticity and reduce economic losses.
2.6 Microscopic examination technology can find the influence of processing methods on feed quality, such as whether the drying method of fishmeal is spray or fermentation, whether the processing of cake flour feed is sufficient, whether it is overheated, etc.
2.7 Make up for the shortcomings of routine chemical analysis. We know that the chemical analysis method cannot explain the composition and source of nutrients of the tested feed. If microscopic inspection technology and chemical analysis are combined in the future, we can learn from each other's strengths and weaknesses and make accurate judgments on feed quality.
For the microscopic inspection of feed, the most important thing is the high-definition observation of the image. The micro-analysis system can restore the image to the computer monitor through the high-definition image sensor and lens. The equipped image analysis software can effectively Processing and analyzing images to help the identification of microorganisms.
1. Image display and conversion
Real-time dynamic observation, capture any visual field image at any time. It has the functions of rotation, enlargement, reduction, mirror image conversion and local observation. It has the functions of cutting, copying, pasting and text input to any area of ​​the image.
2. Target measurement
During the microscopic examination, the observation of the particles in the feed and the measurement of the size are important steps to check whether the feed is adulterated. The system has an online calibration function to achieve accurate measurement (the system has a default calibration value built-in). Accurate measurement of particle diameter, length, radian, angle, arbitrary curve, area, etc.
3. Drawing and annotation
For the opened image, you can draw straight lines, rectangles, circles, and arbitrary curves. Edit the text of the opened image. It is convenient to mark straight lines and angles.
4. Microscopic image analysis system can realize automatic counting of granular cells or particles.
Automatic particle counting can be performed, and the shape parameters such as area, perimeter, diameter, and roundness of each particle can be displayed. Any shape area such as rectangle, circle, umbrella, etc. can be selected for statistics. The diameter range can be set, and a specific size can also be counted. For specific particles, select specific particles based on chroma, brightness, and saturation. Add or delete particles with the mouse click. It is convenient and fast to implement comprehensive statistics on multiple microscopic images.
Shanghai Tiansheng Instrument Co., Ltd. is a high-tech company specialized in microbial analysis and testing technology research and equipment production. At present, it mainly produces biological microscopic analysis systems and other products. Products are widely used in scientific research, inspection and quarantine, quality supervision, environmental monitoring, disease control center, drug and biological product verification, food and pharmaceutical daily chemical production, and iron and petrochemical chemical power.
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