New high performance liquid chromatography mass spectrometry for metabolomics
As one of the concurrent activities of the Shanghai Analytical and Biochemical Exhibition in Munich, the "Chromatography Technology Sino-German Forum" was grandly held on October 16, 2012. The forum is hosted by the Joint Research Center of "Separation and Analysis of Complex Samples". As a good link for Sino-German science and technology exchange, the "Chromatography Technology Sino-German Forum" is held in turn in Munich, Germany and Shanghai, China. In this forum, the latest scientific research progress and hot issues were discussed in depth.
At this forum, researcher Xu Guowang from Dalian Institute of Chemical Physics, Chinese Academy of Sciences gave a wonderful report entitled "New High Performance Liquid Chromatography Mass Spectrometry Based on Metabolomics".
Researcher Xu Guowang, Dalian Institute of Chemical Physics, Chinese Academy of Sciences
Researcher Xu Guowang is engaged in the application of liquid chromatography / capillary electrophoresis in life sciences. He is currently the leader of the Biomolecular High Resolution Separation and Analysis Topic Group of the Biochemical Department of the Dalian Institute of Chemistry, the director of the Metabolomics Research Center, the deputy chairman and secretary general of the Chinese Chromatography Society, the director of the Chinese Chemical Society, and the member of the Science and Technology Committee of the State Tobacco Administration. Since 2003, he has been appointed as the judge of the National Science and Technology Award (Light Industry Group).
The goal of metabolomics is to analyze as many metabolites as possible in the organism. Metabolomics is a useful tool for finding new metabolic markers. The methods commonly used include NMR and MS methods, but so far, due to metabolism The complexity of the group and the defects of various methods, each analysis method can only obtain 10% -15% of the data of the entire metabolic group. For the MS-based method, although the peak capacity can be increased, due to the coexistence of trace metabolites , Lower ionization efficiency and other effects still affect the detection of metabolites. Because high concentrations of metabolites are easily detected, and studies have found that different metabolites produced by different diseases are very similar, these phenomena seriously interfere with the discovery of new metabolic markers.
In order to solve these problems, Xu Guowang's research group has developed a new strategy, including two key points: First, the method reduces the polarity of metabolites and makes MS more sensitive. Second, by using database-based MRM detection methods, targeted metabolome analysis is performed. Through this strategy, the sensitivity and stability of metabolite analysis can be greatly improved.
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