Causes and elimination methods of negative background in ELISA
Causes and elimination methods of negative background in ELISA In enzyme-linked solid-phase immunoassays, especially in the indirect ELISA method, the problem of negative background is often encountered. The main reason is that the background is too high and the background value of different specimens is quite different. Background or background is the non-specific color development in ELISA. The color of the substrate without enzyme action is called blank. If the substrate solution is not properly prepared, a certain blank value will appear. As long as this blank value is not too high (A <0.05), it can be subtracted from each measured value and does not affect the experimental results. What is discussed here is the background of negative specimens that do not contain the test antigen or antibody in ELISA. In theory, only negative specimens can eventually cause color reaction, so why does the background appear? Adsorption of solid-phase carriers: In enzyme immunoassays, ELISA is characterized by the use of means to separate free and bound enzyme conjugates. The solid-phase carrier and the antigen or antibody adsorbed thereon form a solid-phase antigen or a solid-phase antibody, that is, an immunosorbent (Immu-nosorbent). The commonly used solid-phase carrier polystyrene has mainly hydrophobic groups on its surface and binds to proteins through hydrophobic bonds. This physical adsorption is non-specific. Although the molecular weight, isoelectric point, and concentration of the protein have a certain effect on the amount of adsorption, in general, various proteins can be adsorbed on the surface of the polystyrene carrier. Therefore, in addition to purposefully adsorbing the antigen or antibody on the carrier during coating, the adsorption of interfering substances may occur in each step of the ELISA, which eventually leads to color development that is not related to the antigen-antibody reaction. This is The main reason for forming the background.
Computer chair by fabric:
1. Mesh fabric: high-back office chair, Mid-back office chair, Ergonomic adjustable chair.
2. Leather chair: large class chair is most used Leather: genuine leather and imitation leather.
3. Linen fabric:work chair, staff chair, middle class chair.
Computer chair by place of application:
1. Staff chair
2. Working chair
3. Student chair and traning chair
4. Meeting chair
5.Ergonomic chair
6. Mid-back office chair
7. High-back office chair
Office Chair,Office Furniture,Study Room Furniture,Training Conference Furniture
Foshan Hollin Furniture Co.,Ltd , https://www.china-hollin.com