What are the advantages of immunohistochemistry?
1) Specificity The basic principle of strong immunology determines that the binding between antigen and antibody is highly specific. Therefore, in theory, immunohistochemistry is also a specific display of antigen in tissue cells, such as keratin (keratin) shows epithelial Composition, LCA shows lymphocyte composition. Cross-reaction occurs only when there is cross-antigen in tissue cells. (2) High sensitivity In the initial stage of application of immunohistochemistry, due to technical limitations, only direct methods, indirect methods and other low-sensitivity technologies, antibodies at that time can only be diluted several times, dozens of times; Now due to the emergence of ABC method or SP method, antibodies diluted thousands of times, tens of thousands or even billions of times can still bind to antigens in tissue cells. Such highly sensitive antibody antigen reactions make immunohistochemical methods more and more The more convenient it is for routine pathological diagnosis. (3) Accurate positioning, combination of shape and function This technology can accurately locate antigens in tissues and cells through antigen-antibody reaction and color reaction, so it can simultaneously locate and observe different antigens in the same tissue or cell. In this way, the combination of morphology and function can be carried out, which is very meaningful for the in-depth study of pathology. Source: Biotechnology Forum
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