Dilution method-Haibo Bio
2024-10-17 14:00:18
(1) Liquid dilution method
? Firstly make a series of multiple dilutions of the test antibacterial drugs, then add appropriate diluted test bacteria solution to each test tube, shake well, observe after culturing, and use the antimicrobial drug minimum inhibitory concentration (MIC) tube as the sensitivity of the test strain degree. (2) Agar dilution method:
? Add different doses of antibacterial drugs to quantitative agar medium melted and cooled to 45 ℃, mix well, pour into a sterile plate, which is a medium containing decreasing drug concentration;
? Inoculate the test bacteria on this medium, observe the growth of the tested bacteria after cultivation, and the lowest drug inhibits the growth of bacteria, that is, the minimum inhibitory concentration (MIC).
? Firstly make a series of multiple dilutions of the test antibacterial drugs, then add appropriate diluted test bacteria solution to each test tube, shake well, observe after culturing, and use the antimicrobial drug minimum inhibitory concentration (MIC) tube as the sensitivity of the test strain degree. (2) Agar dilution method:
? Add different doses of antibacterial drugs to quantitative agar medium melted and cooled to 45 ℃, mix well, pour into a sterile plate, which is a medium containing decreasing drug concentration;
? Inoculate the test bacteria on this medium, observe the growth of the tested bacteria after cultivation, and the lowest drug inhibits the growth of bacteria, that is, the minimum inhibitory concentration (MIC).
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