Kamaishu (Shanghai) Biotechnology will analyze the main problems of serum culture medium for you
The following article is edited and edited by Kamaishu (Shanghai) Biotechnology:
The main problems of serum medium are summarized as follows:
1. The composition of serum may be as many as hundreds, and its exact composition, content and mechanism of action are still unclear, especially for some of the peptide growth factors, hormones and lipids, etc., which gives Research work brings many difficulties.
2. The sera are produced in batches, which vary greatly from batch to batch, and the storage period of the serum is at most one year. Therefore, it is extremely difficult to ensure the similarity of each batch of serum, which limits the standardization and continuity of the experiment.
3. For most cells, in the body state, serum is not the physiological fluid they are in contact with, only the serum is contacted during injury healing and blood clotting, so the use of serum may change the normal state of certain cells in the body, and serum may promote The growth of certain cells (fibroblasts) simultaneously inhibits the growth of another type of cells (epidermal cells).
4. The serum contains some substances that are toxic to cells, such as polyamine oxidase, and can react with polyamines from highly proliferating cells (such as spermine and spermidine) to form polyspermine with cytotoxic effects. Complements, antibodies, bacterial toxins, etc. can affect cell growth and even cause cell death.
5. Different animals have different origins and batch numbers of serum. The quality of each batch is very different, and its composition cannot be kept the same.
6. Mycoplasma and virus may be brought into the material, which may have a potential impact on the cells, which may lead to experimental failure or unreliable experimental results.
7. In large-scale production, the source of serum is becoming more and more difficult and expensive. It is one of the main parts of the production cost of animal cell culture.
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